Experiment workflows
From scientific question to report
qPCR
Design, execute, analyze, and report qPCR experiments
- • Experiment design
- • Data analysis
- • MIQE-compliant reports
dPCR
PlannedAbsolute quantification with digital PCR technology
- • Counting with Poisson correction
- • Absolute copy numbers
- • Rare variant detection
Tools
Standalone tools for specific tasks
Concentration converter
Convert between mass, molar, and copy number concentrations
- • Mass ↔ Moles ↔ Copies
- • Digital PCR (λ, cpp)
- • Organism templates
Plate homogeneity
Analyze qPCR plate uniformity to validate instrument performance and detect spatial bias
- • Edge effect detection
- • CV & uniformity metrics
- • Exportable reports
Lab workbooks
Download ready-made workbooks or enhance your own
- • Starting templates
- • Interactive tools
- • Visual structure editing
Reference gene finder
Identify the most stable reference genes for qPCR normalization
- • geNorm M-value ranking
- • Optimal gene count (V-value)
- • Ct data import
RDML viewer
NewValidate and visualize RDML (Real-time PCR Data Markup Language) files
- • Schema validation (v1.0–1.3)
- • Metadata, plate layout & Cq table
- • Amplification & melting curves
RDES validator
NewValidate qPCR spreadsheet data against the RDES format for journal submission
- • RDES v1.0 format validation
- • Paste or upload TSV/CSV
- • Actionable error messages
Melt curve prediction
PlannedPredict DNA melt curves and denaturation profiles for PCR amplicons
- • Sequence-based Tm prediction
- • Multi-domain detection
- • Thermodynamic libraries
Resources
Background reading for context